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平臺(tái)編號(hào)：bio-68866
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國(guó)際編號(hào)：CRL-2680?
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細(xì)胞信息： TSHR-T5-51
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
TSHR-T5-51
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
器官來(lái)源：脾
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
年限：adult
品系：BALB/c
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 kappa
ATCC Number：CRL-2680?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)，少量貼壁
規(guī)格：0.5mg Designations: TSHR-T5-51
Depositors: ?H Loosfelt, E Milgrom
Isotype: IgG1 kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor alpha subunit, extracellular domain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Age: adult
Comments:Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody recognizes the N-terminal extracellular domain (amino acids domain 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit).
The TSHR-T5-51 (ATCC CRL-2680 ), TSHR-R5T-44 (ATCC CRL-2681 ) and TSHR-T5U-317 (ATCC CRL-2682 ) antibodies are additive. They recognize different epitopes. TSHR-T5U-317 cross reacts with the rodent TSHR.
The antibodies do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; heat-inactivated horse serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 56150: Loosfelt H, et al. Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295
數(shù)量：大量
器官來(lái)源：脾
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
年限：adult
品系：BALB/c
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 kappa
ATCC Number：CRL-2680?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)，少量貼壁
規(guī)格：0.5mg Designations: TSHR-T5-51
Depositors: ?H Loosfelt, E Milgrom
Isotype: IgG1 kappa
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, with some loosely adherent cells
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: spleen
Strain: BALB/c
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human thyroid stimulating hormone (TSH) receptor alpha subunit, extracellular domain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Age: adult
Comments:Animals were immunized with recombinant human thyroid stimulating hormone (TSH) receptor expressed in. E. coli.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody recognizes the N-terminal extracellular domain (amino acids domain 19-246), alpha subunit of the human thyroid stimulating hormone (TSH) receptor (hormone binding unit).
The TSHR-T5-51 (ATCC CRL-2680 ), TSHR-R5T-44 (ATCC CRL-2681 ) and TSHR-T5U-317 (ATCC CRL-2682 ) antibodies are additive. They recognize different epitopes. TSHR-T5U-317 cross reacts with the rodent TSHR.
The antibodies do not interfere with hormone binding and have no effect on the receptor mediated adenylate cyclase activity.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human TSH receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; heat-inactivated horse serum, 20%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 56150: Loosfelt H, et al. Two-subunit structure of the human thyrotropin receptor. Proc. Natl. Acad. Sci. USA 89: 3765-3769, 1992. PubMed: 1570295
