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平臺(tái)編號(hào)：bio-68870
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國際編號(hào)：CRL-1760?
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細(xì)胞信息： 2C4
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
2C4
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2a
ATCC Number：CRL-1760?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：0.5mg Designations: 2C4
Depositors: ?KL Knight
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against rabbit Ia (cytotoxic)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with rabbit spleen cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with an isotypic determinant present on all rabbit Ia molecules, and precipitates polypeptides with molecular weights of 28000, 31000 and 35000 daltons (corresponding to the beta, Ii and alpha chains, respectively).
The antibody is cytotoxic, inhibits mixed lymphocyte reactivity by 80% and inhibits responses to LPS (but not to Con A or PHA).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1314: Lobel SA, Knight KL. The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody. Immunology 51: 35-43, 1984. PubMed: 6197360
免疫類型：IgG2a
ATCC Number：CRL-1760?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：0.5mg Designations: 2C4
Depositors: ?KL Knight
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against rabbit Ia (cytotoxic)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with rabbit spleen cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with an isotypic determinant present on all rabbit Ia molecules, and precipitates polypeptides with molecular weights of 28000, 31000 and 35000 daltons (corresponding to the beta, Ii and alpha chains, respectively).
The antibody is cytotoxic, inhibits mixed lymphocyte reactivity by 80% and inhibits responses to LPS (but not to Con A or PHA).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium, 85%; horse serum, 10%; fetal bovine serum, 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1314: Lobel SA, Knight KL. The role of rabbit Ia molecules in immune functions as determined with the use of an anti-Ia monoclonal antibody. Immunology 51: 35-43, 1984. PubMed: 6197360
