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平臺(tái)編號(hào)：bio-68874
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國(guó)際編號(hào)：CRL-2226?
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細(xì)胞信息： 2D10
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
2D10
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
年限：adult
ATCC Number：CRL-2226?
品系：Lewis
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：其他
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
規(guī)格：0.5mg Designations: 2D10
Depositors: ?SE Buchholz
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: lymph node, popliteal
Strain: Lewis
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse B7.2
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Comments:Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody will neutralize the B7.2 antigen, but does not react with the B7.1 antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22286: Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723
22935: Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442
23485: Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. PubMed: 7510738
免疫類型：IgG2b
年限：adult
ATCC Number：CRL-2226?
品系：Lewis
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：其他
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
規(guī)格：0.5mg Designations: 2D10
Depositors: ?SE Buchholz
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Organ: lymph node, popliteal
Strain: Lewis
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse B7.2
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: adult
Comments:Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody will neutralize the B7.2 antigen, but does not react with the B7.1 antigen.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
- 0.05 mM 2-mercaptoethanol
- fetal bovine serum to a final concentration of 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22286: Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723
22935: Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442
23485: Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. PubMed: 7510738
