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平臺編號：bio-68880
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國際編號：CRL-1741?
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細胞信息： Y13-238
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
Y13-238
細胞形態(tài)：淋巴樣
免疫類型：IgG2a
ATCC Number：CRL-1741?
運輸方式：凍存運輸
數量：大量
是否是腫瘤細胞：0
物種來源：褐鼠
生長狀態(tài)：混合型生長
規(guī)格：0.5mg Designations: Y13-238
Depositors: ?JR Stephenson, ME Furth
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed, adherent and suspension
Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Rats were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.
Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.
The antibody is specific for the Ha-ras (Harvey) p21 and does not react with the p21 of Ki-ras (Kirsten).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Maintain the cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow the density to exceed 1 X 10(6) cells/ml.
Subculturing: Protocol: Dislodge attached cells from the substrate with a cell scraper, aspirate and dispense into new flasks.
Medium Renewal: two to three times weekly
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982. PubMed: 6287003
免疫類型：IgG2a
ATCC Number：CRL-1741?
運輸方式：凍存運輸
數量：大量
是否是腫瘤細胞：0
物種來源：褐鼠
生長狀態(tài)：混合型生長
規(guī)格：0.5mg Designations: Y13-238
Depositors: ?JR Stephenson, ME Furth
Isotype: IgG2a
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed, adherent and suspension
Organism: Rattus norvegicus (B cell); Rattus norvegicus (myeloma) deposited as rat (B cell); rat (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the p21 transforming protein encoded by the v-ras and c-ras (ras) oncogenes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Rats were immunized with syngeneic normal rat kidney cells (NRK) that had been infected with Harvey murine sarcoma virus.
Spleen cells were fused with 210RCY3 Ag 1.2.3 myeloma cells.
The antibody is specific for the Ha-ras (Harvey) p21 and does not react with the p21 of Ki-ras (Kirsten).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Maintain the cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Do not allow the density to exceed 1 X 10(6) cells/ml.
Subculturing: Protocol: Dislodge attached cells from the substrate with a cell scraper, aspirate and dispense into new flasks.
Medium Renewal: two to three times weekly
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1320: Furth ME, et al. Monoclonal antibodies to the p21 products of the transforming gene of Harvey murine sarcoma virus and of the cellular ras gene family. J. Virol. 43: 294-304, 1982. PubMed: 6287003
