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品系：C3H/HeJ
ATCC Number：CRL-2168?
數(shù)量：大量
組織來(lái)源：vascular epithelium
細(xì)胞形態(tài)：上皮樣
細(xì)胞類型：SV40轉(zhuǎn)化細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來(lái)源：腋窩淋巴結(jié)
運(yùn)輸方式：凍存運(yùn)輸
年限：adult
規(guī)格：0.5mg Designations: 2F-2B
Depositors: ?KA O'Connell
Biosafety Level:2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:epithelial


Source: Organ: axillary lymph node
Strain: C3H/HeJ
Tissue: vascular epithelium
Cell Type: endothelialSV40 transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:H-2 K; VCAM
Age: adult
Gender: male
Comments:The 2F-2B cell line was derived from a nodular cutaneous solid tumor in nude mice injected with SVEC4-10EE2 (see ATCC CRL- 2167). The line was cloned in 1991 by limiting dilution. The cells are sensitive in vitro to both macrophages and to natural killer (NK) cells.They express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and vascular cell adhesion molecule (VCAM).The cells stain positively for SV40 T antigen.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170
23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612
23187: O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299