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2T8-3E10
    2T8-3E10
  • 平臺(tái)編號(hào):bio-68898
  • 國(guó)際編號(hào):HB-8213?
  • 細(xì)胞信息: 2T8-3E10
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購(gòu)
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫(kù)為準(zhǔn))

ATCC Number:HB-8213?
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來源:小鼠
數(shù)量:大量
細(xì)胞形態(tài):淋巴樣
免疫類型:IgM
規(guī)格:0.5mg Designations: 2T8-3E10
Depositors: ?Dana Farber Cancer Inst.
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human T cell antigen (Ti1b antigen) expressed only on the CT8III cytotoxic T lymphocyte clone
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with CT8III cells.
Spleen cells were fused with NS-1 myeloma cells.
The antibody does not react with human thymocytes or resting or activated peripheral T cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 64%; NCTC 109 medium, 10%; L-glutamine, 2%; sodium pyruvate, 1%; hypoxanthine-thymidine, 1%; oxaloacetic acid and insulin, 1%; horse serum, 10%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3546: Schlossman SF, et al. Monoclonal antibodies that recognize human T cells. US Patent 4,550,086 dated Oct 29 1985

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