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平臺(tái)編號(hào)：bio-68902
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國(guó)際編號(hào)：TIB-203?
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細(xì)胞信息： 2PK-3
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
2PK-3
細(xì)胞形態(tài)：淋巴樣
免疫類(lèi)型：IgG2 kappa , IgG2; kappa light chain
細(xì)胞類(lèi)型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
品系：BALB/c
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：TIB-203?
相關(guān)疾?。毫馨土?br /> 規(guī)格：0.5mg Designations: 2PK-3
Depositors: ?NL Warner
Isotype: IgG2 kappa , IgG2; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products:surface immunoglobulin (sIg +)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:I-A d; I-E d
Comments:Derived from a lymphoma induced with mineral oil and casein in a BALB/c mouse.
It is representative of a mature B lymphocyte just prior to the plasma cell stage of differentiation.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 21886: . Membrane receptors of lymphocytes. Amsterdam: Elsevier/North Holland; 1975.
22846: Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906
23536: Lanier LL, et al. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J. Immunol. 127: 1691-1697, 1981. PubMed: 7024413
32236: Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620
58054: Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803
免疫類(lèi)型：IgG2 kappa , IgG2; kappa light chain
細(xì)胞類(lèi)型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
品系：BALB/c
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
ATCC Number：TIB-203?
相關(guān)疾?。毫馨土?br /> 規(guī)格：0.5mg Designations: 2PK-3
Depositors: ?NL Warner
Isotype: IgG2 kappa , IgG2; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products:surface immunoglobulin (sIg +)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:I-A d; I-E d
Comments:Derived from a lymphoma induced with mineral oil and casein in a BALB/c mouse.
It is representative of a mature B lymphocyte just prior to the plasma cell stage of differentiation.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 21886: . Membrane receptors of lymphocytes. Amsterdam: Elsevier/North Holland; 1975.
22846: Warner NL, et al. Flow cytometry analysis of murine B cell lymphoma differentiation. Immunol. Rev. 48: 197-243, 1979. PubMed: 161906
23536: Lanier LL, et al. Quantitative immunofluorescent analysis of surface phenotypes of murine B cell lymphomas and plasmacytomas with monoclonal antibodies. J. Immunol. 127: 1691-1697, 1981. PubMed: 7024413
32236: Brumeanu TD, et al. Immunogenicity of a contiguous T-B synthetic epitope of the A/PR/8/34 influenza virus. J. Virol. 71: 5473-5480, 1997. PubMed: 9188620
58054: Gutman GA, et al. Immunoglobulin production by murine B-lymphoma cells. Clin. Immunol. Immunopathol. 18: 230-244, 1981. PubMed: 6781803
