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平臺編號：bio-68916
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國際編號：CRL-1591?
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細胞信息： TRA-171
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
TRA-171
生長狀態(tài)：貼壁生長
年限：larva
運輸方式：凍存運輸
器官來源：其他
細胞形態(tài)：成纖維樣
ATCC Number：CRL-1591?
是否是腫瘤細胞：0
物種來源：其他
數(shù)量：大量
規(guī)格：0.5mg Designations: TRA-171
Depositors: ?G Kuno
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Toxorhynchites amboinensis
Morphology:fibroblast
Source: Organ: larva, whole
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility:dengue virus types 1, 2, and 3; laboratory adapted dengue virus types 1, 2, 3, and 4
Age: larva
Propagation: ATCC complete growth medium: MThe base medium for this cell line is a 1:1 mixture of MM (Mitsuhashi and Maramorosch) and VP12 Medium (J. Med. Entomol. 6:432-439, 1969. PubMed: 5360492) To make the complete growth medium, add the following components to the base medium:
heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 28.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 28 hrs
References: 22662: Kuno G. New cell line. A continuous cell line of a nonhematophagous mosquito, Toxorhynchites amboinensis. In Vitro 16: 915-917, 1980.
26097: Varma MG, Pudney M. The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432-439, 1969. PubMed: 5360492
年限：larva
運輸方式：凍存運輸
器官來源：其他
細胞形態(tài)：成纖維樣
ATCC Number：CRL-1591?
是否是腫瘤細胞：0
物種來源：其他
數(shù)量：大量
規(guī)格：0.5mg Designations: TRA-171
Depositors: ?G Kuno
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Toxorhynchites amboinensis
Morphology:fibroblast
Source: Organ: larva, whole
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility:dengue virus types 1, 2, and 3; laboratory adapted dengue virus types 1, 2, 3, and 4
Age: larva
Propagation: ATCC complete growth medium: MThe base medium for this cell line is a 1:1 mixture of MM (Mitsuhashi and Maramorosch) and VP12 Medium (J. Med. Entomol. 6:432-439, 1969. PubMed: 5360492) To make the complete growth medium, add the following components to the base medium:
Temperature: 28.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 28 hrs
References: 22662: Kuno G. New cell line. A continuous cell line of a nonhematophagous mosquito, Toxorhynchites amboinensis. In Vitro 16: 915-917, 1980.
26097: Varma MG, Pudney M. The growth and serial passage of cell lines from Aedes aegypti (L.) larvae in different media. J. Med. Entomol. 6: 432-439, 1969. PubMed: 5360492
