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平臺(tái)編號(hào)：bio-68923
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國(guó)際編號(hào)：HB-254?
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細(xì)胞信息： GL7
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
GL7
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：HB-254?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM
規(guī)格：0.5mg Designations: GL7
Depositors: ?G Lazlo, KS Hathcock
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse lymphocytes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with LPS activated DBA/2J mouse B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells.
The GL7 antigen is expressed on up to 70% of activated B cells and on high proportions of ConA activated CD4 + and CD8 + T cells.
Unstimulated B and T cells are infrequently GL7 positive.
The antigen appears to be a lymphocyte activation antigen that is different from those previously identified.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 1% nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23482: Laszlo G, et al. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252-5262, 1993. PubMed: 8515058
物種來(lái)源：大鼠
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：HB-254?
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgM
規(guī)格：0.5mg Designations: GL7
Depositors: ?G Lazlo, KS Hathcock
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against mouse lymphocytes
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with LPS activated DBA/2J mouse B cells.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with a 35000 dalton antigen present on subsets of activated mouse B cells and activated CD4 + and CD 8 + mouse T cells.
The GL7 antigen is expressed on up to 70% of activated B cells and on high proportions of ConA activated CD4 + and CD8 + T cells.
Unstimulated B and T cells are infrequently GL7 positive.
The antigen appears to be a lymphocyte activation antigen that is different from those previously identified.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 1% nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23482: Laszlo G, et al. Characterization of a novel cell-surface molecule expressed on subpopulations of activated T and B cells. J. Immunol. 150: 5252-5262, 1993. PubMed: 8515058
