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品系：LT/Sv 

是否是腫瘤細(xì)胞：0 

物種來源：小鼠 

細(xì)胞形態(tài)：上皮樣 

ATCC Number：CRL-2389

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年限：12 month old 

運(yùn)輸方式：凍存運(yùn)輸 

器官來源：胰腺 

生長(zhǎng)狀態(tài)：貼壁生長(zhǎng) 

數(shù)量：大量 

規(guī)格：0.5mg                                                                                                                 Designations 別名 : LTPA 

Depositors:EH Leiter 

Biosafety Level 生物安全等級(jí) :1 

Shipped 提供形式 :frozen         

Medium & Serum: See Propagation 

Growth Properties:adherent 

Organism:Mus musculus deposited as Mus musculus  (mouse)

Morphology:epithelial 

Source:   

Organ: pancreas    

Strain:  LT/Sv    

Disease: adenocarcinoma

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits  may be required for the     transfer of this ATCC material. 

Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.Please click here  for information regarding the specific requirements for shipment to your location.

Cytogenetic  

Analysis:Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%

Age:12 month old 

Gender:female    

Comments 注釋 :LTPA is an epithelial cell line derived in 1975 by Edward H.Leiter at The Jackson Laboratory,Bar Harbor, Maine from a spontaneous pancreatic adenocarcinoma taken from a 12 month old female Lt/Sv mouse. 

Adefective Polyoma virus infection of a pancreatic duct cell or its precursor appears to represent the neoplastic transforming event. LTPA cells carry a persistent Polyoma infection.

When injected subcutaneously into Swiss nu/nu mice, LTPA cells formed ductular structures, which were destroyed by inflammatory reactions within 3 weeks. A culture submitted to the ATCC in May of 1998 was found to be contaminated with mycoplasma and progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma,by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. 

All tests were negative.   

Propagation:ATCC complete growth medium:The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003.  

To make the complete growth medium, add the following components to the base medium:  fetal bovine serum to a final concentration of 10%.

Temperature 培養(yǎng)溫度 : 37.0°C   

Subculturing:

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended 

Medium Renewal: Every 2 to 3 days Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room emperature (or at 37C) until the cells detach. 

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation:culture medium 95%; DMSO, 5%   

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003 recommended serum:ATCC 30-2020 

References 參考文獻(xiàn) :38749: Leiter EH, et al.An epithelial cell line with chronic polyoma infection established from a spontaneous mouse pancreatic adenocarcinoma.Cancer Res. 38:969-977, 1978. PubMed: 205354