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數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b; kappa light chain
ATCC Number：CRL-1960?
規(guī)格：0.5mg Designations: 2B5.3
Depositors: ?MF Heyworth
Isotype: IgG2b; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Giardia muris trophozoites
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with G. muris trophozoites.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody reacts with G. muris trophozoite surfaces.
Unlike 3C7.2 (ATCC CRL-1959 ), 1A3.1 (ATCC CRL-1961 ) and 1B1.1, the 2B5.3 antibody specifically recognizes trophozoite antigens of apparent molecular weight 31000 and 35000 on western blots.
The antibody immunoprecipitates two polypeptides with apparent molecular weights of 30000.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%;NCTC 109 medium, 10%;(Add 1 mM oxaloacetic acid, 0.45 mM sodium pyruvate, 4 mM L-glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine and 10 units/ml bovine insulin to this mixture);horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22728: Heyworth MF, et al. Generation and characterization of monoclonal antibodies against Giardia muris trophozoites. Immunology 68: 341-345, 1989. PubMed: 2592009