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平臺編號：bio-68940
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國際編號：ATCC CRL-1715
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細胞信息： TM4
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
TM4
年限：11 to 13 days
數(shù)量：大量
運輸方式：凍存運輸
ATCC Number：CRL-1715
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器官來源：睪丸
細胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：小鼠
規(guī)格：0.5mg Designations 菌種別名 : TM4
Depositors:JP Mather
Biosafety Level 生物安全等級 :1
Shipped 提供形式 :frozen
Medium & Serum: See Propagation
Growth Properties:adherent
Organism:Mus musculus
Morphology:epithelial
Source:
Organ: testis
Disease: normal
Cell Type:
Sertoli cell;
Cellular Products:retinol binding protein tissue plasminogen activator transferrin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.
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Applications:transfection host
Receptors:follicle stimulating hormone (FSH),expressed androgen receptor, expressed progesterone receptor, expressed Tumorigenic:No
Antigen
Expression:H-Y antigen; Mus musculus, expressed
Age:11 to 13 days
Gender:male
Comments 注釋 :The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH).
The FSH responsiveness is much reduced compared to primary sertoli cell cultures.
Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid. Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: A 1:1 mixture of Ham'S F12 medium and Dulbecco's modified Eagle's medium with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Atmosphere 需氧情況 : air, 95%; carbon dioxide (CO2),5%
Temperature 培養(yǎng)溫度 : 37.0°C
Subculturing:
Protocol:Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell supension to new culture vessels. Incubate cultures at 37C.
Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 3 to 4 days
Preservation:
Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related
Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040
References 參考文獻 : 1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781
1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium.Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561
1184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259:3117-3123, 1984. PubMed: 6538197
26150: Mather JP, Phillips DM.Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture.J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874
