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器官來(lái)源：肺
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
年限：403 days
是否是腫瘤細(xì)胞：0
物種來(lái)源：其他
ATCC Number：CCL-100?
規(guī)格：0.5mg Designations: GeLu
Depositors: ?ML Macy
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Meriones unguiculatus
Morphology:fibroblast


Source: Organ: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1, 3
Age: 403 days
Gender: female
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin - 0.53 mM EDTA solution and remove. Place culture at room temperature until cells detach (approximately 10 to 20 minutes). Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 26276: . . TCA (Tissue Cult. Assoc.) Man. 3: 617-621, 1977.