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平臺(tái)編號(hào)：bio-68952
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國(guó)際編號(hào)：HB-8178?
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細(xì)胞信息： 2BD4E4 K99
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
2BD4E4 K99
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：HB-8178?
細(xì)胞形態(tài)：淋巴樣
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
免疫類(lèi)型：IgG1; kappa light chain
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
規(guī)格：0.5mg Designations: 2BD4E4 K99
Depositors: ?Molecular Genetics, Inc.
Isotype: IgG1; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Escherichia coli K99 pilus
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with E. coli K99 pilus.
Spleen cells were fused with NS-1 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 15%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1005: Chan R, et al. Use of specific antibody to demonstrate glycocalyx, K99 pili and the spatial relationships of K99 & enterotoxigenic Escherichia coli in the ileum of colostrum-fed calves. Infect. Immun. 37: 1170-1180, 1982. PubMed: 6127313
3513: Sadowski PL. Production of monoclonal antibodies against bacterial adhesins. US Patent 4,443,549 dated Apr 17 1984
ATCC Number：HB-8178?
細(xì)胞形態(tài)：淋巴樣
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
免疫類(lèi)型：IgG1; kappa light chain
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
規(guī)格：0.5mg Designations: 2BD4E4 K99
Depositors: ?Molecular Genetics, Inc.
Isotype: IgG1; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Escherichia coli K99 pilus
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with E. coli K99 pilus.
Spleen cells were fused with NS-1 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1005: Chan R, et al. Use of specific antibody to demonstrate glycocalyx, K99 pili and the spatial relationships of K99 & enterotoxigenic Escherichia coli in the ileum of colostrum-fed calves. Infect. Immun. 37: 1170-1180, 1982. PubMed: 6127313
3513: Sadowski PL. Production of monoclonal antibodies against bacterial adhesins. US Patent 4,443,549 dated Apr 17 1984
