細(xì)胞類型:T淋巴細(xì)胞
數(shù)量:大量
是否是腫瘤細(xì)胞:0
物種來源:小鼠
運輸方式:凍存運輸
細(xì)胞形態(tài):淋巴樣
品系:C57BL/6
ATCC Number:TIB-38?
相關(guān)疾病:淋巴瘤
生長狀態(tài):懸浮生長
規(guī)格:0.5mg Designations: TIMI.4G.1.3
Depositors: ?R Hyman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2b; Thy-1.2
Comments:This line was derived from TIMI.4 (ATCC TIB-37 ).
The cells are resistant to 0.1 mM 6-thioguanine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1129: Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114