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平臺編號：bio-68963
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國際編號：HB-11911?
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細(xì)胞信息： 127H
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用及說明書：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
127H
是否是腫瘤細(xì)胞：0
物種來源：小鼠
免疫類型：IgG1
ATCC Number：HB-11911?
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
生長狀態(tài)：懸浮生長
規(guī)格：0.5mg Designations: 127H
Depositors: ?ICOS Corporation
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Cellular Products:immunoglobulin; monoclonal antibody; against rat ICAM-4
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Mice were immunized with the purified recombinant rat ICAM-4, domains 1-6 protein.
Spleen cells were fused with NS-1 myeloma cells.
Monoclonal antibody 127A (ATCC HB-11905 ) recognized the D1/GST fusion protein and 127H (ATCC HB-11911 ) recognized the D2/GST fusion protein.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Maintain cultures at cell concentrations between 2 X 10exp5 and 6 X 10exp5 viable cells/ml.
References: 40119: Kilgannon PD, Gallatin M. Anti-ICAM-4 antibodies and hybridomas. US Patent 5,773,293 dated Jun 30 1998
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