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數(shù)量：大量
器官來源：皮膚
年限：63 years
ATCC Number：CRL-1326?
相關(guān)疾?。篍hlers-Danlos綜合征子
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：0
物種來源：人
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.5mg Designations: Gam Per
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: skin
Disease: Ehlers-Danlos syndrome, possible heterozygote
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 63 years
Gender: female
Comments:The tissue donor was a possible heterozygote for Ehlers-Danlos syndrome, and was the grandmother of Jo Per (ATCC CRL-1332 ), No Per (ATCC CRL-1327 ) and Cor Per II (ATCC CRL-1243).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 8 to 10 days.