數(shù)量:大量
運輸方式:凍存運輸
是否是腫瘤細胞:0
物種來源:小鼠
生長狀態(tài):懸浮生長
細胞形態(tài):淋巴樣
免疫類型:IgA
ATCC Number:HB-8087?
規(guī)格:0.5mg Designations: 129A3/1
Depositors: ?Johns Hopkins University
Isotype: IgA
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:recommended serum:ATCC 30-2020
References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. US Patent 4,416,866 dated Nov 22 1983