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平臺(tái)編號(hào)：bio-68990
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國(guó)際編號(hào)：CRL-2760?
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細(xì)胞信息： G14D
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
G14D
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：其他
器官來源：外周血
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)，多細(xì)胞聚集
品系：Marion
ATCC Number：CRL-2760?
規(guī)格：0.5mg Designations: G14D
Depositors: ?NW Miller
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates
Organism: Ictalurus punctatus
Morphology:lymphoblast
Source: Strain: Marion
Organ: peripheral blood
Cell Type: lymphocyte T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1998
Receptors:T cell antigen receptor, expressed
Cytogenetic Analysis:diploid
Comments:The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors.
Propagation: ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Fetal Bovine Serum supplented with 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
References: 61263: Hogan RJ, et al. Molecular and Immunologic Characterization of Gynogenetic Channel Catfish (Ictalurus punctatus). Mar. Biotechnol. 1: 317-327, 1999. PubMed: 10489406
細(xì)胞形態(tài)：淋巴樣
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：其他
器官來源：外周血
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)，多細(xì)胞聚集
品系：Marion
ATCC Number：CRL-2760?
規(guī)格：0.5mg Designations: G14D
Depositors: ?NW Miller
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates
Organism: Ictalurus punctatus
Morphology:lymphoblast
Source: Strain: Marion
Organ: peripheral blood
Cell Type: lymphocyte T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1998
Receptors:T cell antigen receptor, expressed
Cytogenetic Analysis:diploid
Comments:The G14D cell line developed in 1998 is an immortal homozygous T cell line spontaneously derived by in vitro lipopolysaccharide (LPS) stimulation of peripheral blood from a normal gynogenetic channel catfish.. The cells were, subsequently, cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells constitutively produce interleukin-like factors.
Propagation: ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 10% double distilled water and 5% heat-inactivated catfish serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml. Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Fetal Bovine Serum supplented with 7.5% DMSO
Storage temperature: liquid nitrogen vapor phase
References: 61263: Hogan RJ, et al. Molecular and Immunologic Characterization of Gynogenetic Channel Catfish (Ictalurus punctatus). Mar. Biotechnol. 1: 317-327, 1999. PubMed: 10489406
