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平臺編號：bio-69000
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國際編號：CRL-2136?
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細(xì)胞信息： TGP49
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
TGP49
生長狀態(tài)：貼壁生長
品系：Tg(E1a-1-SV40E)Bri18
年限：26 weeks
器官來源：胰腺
是否是腫瘤細(xì)胞：0
物種來源：轉(zhuǎn)基因小鼠
ATCC Number：CRL-2136?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。浩渌膊?br /> 細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
規(guī)格：0.5mg Designations: TGP49
Depositors: ?OS Pettengill, D Longnecker
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus , transgenic deposited as mouse, transgenic
Morphology:epithelial
Source: Organ: pancreas
Strain: Tg(E1a-1-SV40E)Bri18
Disease: pancreatic acinar cell tumor; carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
Age: 26 weeks
Gender: male
Comments:TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin.
Current stocks of TGP49 are negative for somatostatin and positive for trypsin. The cells do not secrete amylase or lipase. Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22323: Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904
品系：Tg(E1a-1-SV40E)Bri18
年限：26 weeks
器官來源：胰腺
是否是腫瘤細(xì)胞：0
物種來源：轉(zhuǎn)基因小鼠
ATCC Number：CRL-2136?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。浩渌膊?br /> 細(xì)胞形態(tài)：上皮樣
數(shù)量：大量
規(guī)格：0.5mg Designations: TGP49
Depositors: ?OS Pettengill, D Longnecker
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus , transgenic deposited as mouse, transgenic
Morphology:epithelial
Source: Organ: pancreas
Strain: Tg(E1a-1-SV40E)Bri18
Disease: pancreatic acinar cell tumor; carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
Age: 26 weeks
Gender: male
Comments:TGP49 is an epithelial-like cell line derived from a pancreatic tumor with acinar cell morphology that arose in an adult male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
In culture, TGP49 cells lost acinar cell morphology and transiently produced somatostatin.
Current stocks of TGP49 are negative for somatostatin and positive for trypsin. The cells do not secrete amylase or lipase. Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22323: Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. PubMed: 7904904
