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細胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
ATCC Number：CRL-2118?
相關疾?。焊伟?br /> 品系：Leghorn
運輸方式：凍存運輸
數(shù)量：大量
器官來源：肝
是否是腫瘤細胞：0
物種來源：其他
規(guī)格：0.5mg Designations: LMH/2A
Depositors: ?R Binder, DL Williams
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Gallus gallus
Morphology:epithelial


Source: Organ: liver
Strain: Leghorn
Disease: hepatocellular carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:yolk protein genes, and permits the analysis of estrogen responsive genes involved in the transport of yolk proteins and other nutrients to the oocyte.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
Vessels prepared in this fashion may be used immediately.
Receptors:estrogen receptor, positive
Tumorigenic:Yes
Cytogenetic Analysis:triploid; modal number = 116; six marker chromosomes
Comments:LMH/2A is a stably transfected cell line constructed in 1989 by R. Binder from the LMH hepatocellular carcinoma cell line (see ATCC CRL-2117 ).
By cotransfection with the plasmid pRSVCEO (containing the chicken estrogen receptor gene driven by the Rous sarcoma promoter) and the pRSVNEO plasmid (which confers G418 resistance).
LMH/2A cells express high levels of chicken estrogen receptor.
Apolipoprotein (apoII) gene expression is increased by estrogen 300-fold compared with levels in the low receptor expression parent LMH line.
This cell line is estrogen responsive for the expression of endogenous
yolk protein genes, and permits the analysis of estrogen responsive genes involved in the transport of yolk proteins and other nutrients to the oocyte.
These genes include apolipoprotein B and apoII as well as vitellogenin.
NOTE: tissue culture vessels used to propagate this line must be precoated with 0.1% gelatin.
To prepare the vessels, flood them with gelatin solution, refrigerate for 5 to 10 minutes, and remove the liquid.
Vessels prepared in this fashion may be used immediately.
Propagation: ATCC complete growth medium: Waymouth's MB 752/1 medium, 90%; fetal bovine serum, 10%
Temperature: 37.0℃
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Flasks must be coated with 0.1% gelatin.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22374: Binder R, et al. Expression of endogenous and transfected apolipoprotein II and vitellogenin II genes in an estrogen responsive chicken liver cell line. Mol. Endocrinol. 4: 201-208, 1990. PubMed: 2330000
23063: Kawaguchi T, et al. Establishment and characterization of a chicken hepatocellular carcinoma cell line, LMH. Cancer Res. 47: 4460-4463, 1987. PubMed: 3607775
23244: Sensel MG, et al. Reactivation of apolipoprotein II gene transcription by cycloheximide reveals two steps in the deactivation of estrogen receptor-mediated transcription. Mol. Cell. Biol. 14: 1733-1742, 1994. PubMed: 8114707