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平臺編號：bio-69005
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國際編號：HB-8086?
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細胞信息： 132C4A/4
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
132C4A/4
細胞形態(tài)：淋巴樣
免疫類型：IgG1
運輸方式：凍存運輸
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：HB-8086?
生長狀態(tài)：懸浮生長
規(guī)格：0.5mg Designations: 132C4A/4
Depositors: ?Johns Hopkins University
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.2 units/ml insulin, 1 mM sodium pyruvate, 0.15 mg/ml oxaloacetic acid, 0.01 M HEPES and HAT, 70%; NCTC 135 medium, 10%; heat- inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 40%; fetal bovine serum, 50%; DMSO, 10%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. US Patent 4,416,866 dated Nov 22 1983
免疫類型：IgG1
運輸方式：凍存運輸
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：HB-8086?
生長狀態(tài)：懸浮生長
規(guī)格：0.5mg Designations: 132C4A/4
Depositors: ?Johns Hopkins University
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against surface (cercariae) glycoprotein on Schistosoma mansoni
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with cercariae glycoprotein from Schistosoma mansoni.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 0.2 units/ml insulin, 1 mM sodium pyruvate, 0.15 mg/ml oxaloacetic acid, 0.01 M HEPES and HAT, 70%; NCTC 135 medium, 10%; heat- inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 40%; fetal bovine serum, 50%; DMSO, 10%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 3511: Strand M. Diagnosis and treatment of fluke infections with monoclonal antibodies. US Patent 4,416,866 dated Nov 22 1983
