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平臺編號：bio-69011
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國際編號：CRL-1447?
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細(xì)胞信息： G-7
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
G-7
品系：Swiss
數(shù)量：大量
組織來源：skeletal muscle
ATCC Number：CRL-1447?
細(xì)胞形態(tài)：成肌細(xì)胞
生長狀態(tài)：貼壁生長
細(xì)胞類型：其他細(xì)胞類型
年限：fetus fetus
是否是腫瘤細(xì)胞：0
物種來源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5mg Designations: G-7
Depositors: ?J Peacock
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:myoblast
Source: Strain: Swiss
Tissue: skeletal muscle
Cell Type: myoblast myoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Cytogenetic Analysis:modal number = 40
Age: fetus fetus
Comments:When confluent, the cells fuse to form multinucleated myotubes.
They are slightly responsive to acetylcholine.
The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, remove trypsin, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks. Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Doubling Time: 17 to 19 hrs
References: 1063: Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
數(shù)量：大量
組織來源：skeletal muscle
ATCC Number：CRL-1447?
細(xì)胞形態(tài)：成肌細(xì)胞
生長狀態(tài)：貼壁生長
細(xì)胞類型：其他細(xì)胞類型
年限：fetus fetus
是否是腫瘤細(xì)胞：0
物種來源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5mg Designations: G-7
Depositors: ?J Peacock
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:myoblast
Source: Strain: Swiss
Tissue: skeletal muscle
Cell Type: myoblast myoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Cytogenetic Analysis:modal number = 40
Age: fetus fetus
Comments:When confluent, the cells fuse to form multinucleated myotubes.
They are slightly responsive to acetylcholine.
The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, remove trypsin, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks. Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Doubling Time: 17 to 19 hrs
References: 1063: Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
