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平臺(tái)編號(hào)：bio-69015
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國(guó)際編號(hào)：CRL-1666?
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細(xì)胞信息： 13762 MAT B III
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
13762 MAT B III
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
運(yùn)輸方式：凍存運(yùn)輸
品系：Fischer 344 (F344)
器官來(lái)源：乳腺
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
ATCC Number：CRL-1666?
相關(guān)疾?。合侔?br /> 數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
規(guī)格：0.5mg Designations: 13762 MAT B III
Depositors: ?AE Bogden
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension or loosely adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: mammary gland
Strain: Fischer 344 (F344)
Disease: adenocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The 13762 MAT B III cell line was established at the EG&G Mason Research Institute from a transplantable rat ascites tumor derived from the 13762 solid mammary adenocarcinoma.
Prior to its deposit, the cell line was tested and found negative for the following viruses:
Pneumonia virus of mice (PVM), reovirus type 3 (Reo3), K virus, polyomavirus, Sendai virus, GDVII (mouse poliovirus), Kilham rat virus (KRV), minute virus of mice (MVM), SV5, H-1 virus and ectromelia virus.
The line grows as small clusters of rounded cells lightly attached to the substrate, or as a static suspension.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at 1 X 10 exp5/ml and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 32293: Xing RH, et al. Prevention of breast cancer growth, invasion, and metastasis by antiestrogen tamoxifen alone or in combination with urokinase inhibitor B-428. Cancer Res. 57: 3585-3593, 1997. PubMed: 9270032
運(yùn)輸方式：凍存運(yùn)輸
品系：Fischer 344 (F344)
器官來(lái)源：乳腺
是否是腫瘤細(xì)胞：0
物種來(lái)源：大鼠
ATCC Number：CRL-1666?
相關(guān)疾?。合侔?br /> 數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
規(guī)格：0.5mg Designations: 13762 MAT B III
Depositors: ?AE Bogden
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension or loosely adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:epithelial
Source: Organ: mammary gland
Strain: Fischer 344 (F344)
Disease: adenocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:The 13762 MAT B III cell line was established at the EG&G Mason Research Institute from a transplantable rat ascites tumor derived from the 13762 solid mammary adenocarcinoma.
Prior to its deposit, the cell line was tested and found negative for the following viruses:
Pneumonia virus of mice (PVM), reovirus type 3 (Reo3), K virus, polyomavirus, Sendai virus, GDVII (mouse poliovirus), Kilham rat virus (KRV), minute virus of mice (MVM), SV5, H-1 virus and ectromelia virus.
The line grows as small clusters of rounded cells lightly attached to the substrate, or as a static suspension.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at 1 X 10 exp5/ml and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 32293: Xing RH, et al. Prevention of breast cancer growth, invasion, and metastasis by antiestrogen tamoxifen alone or in combination with urokinase inhibitor B-428. Cancer Res. 57: 3585-3593, 1997. PubMed: 9270032
