器官來(lái)源:肺
運(yùn)輸方式:凍存運(yùn)輸
細(xì)胞類型:成纖維細(xì)胞
細(xì)胞形態(tài):成纖維樣
是否是腫瘤細(xì)胞:0
物種來(lái)源:人
ATCC Number:CCL-191?
相關(guān)疾?。浩渌膊?br />
年限:48 years
數(shù)量:大量
生長(zhǎng)狀態(tài):貼壁生長(zhǎng)
規(guī)格:0.5mg Designations: LL 97A (AlMy)
Depositors: ?RG Crystal
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Disease: idiopathic pulmonary fibrosis
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,13
D16S539: 11,13
D5S818: 13
D7S820: 9,11
THO1: 6,9.3
TPOX: 10,11
vWA: 14,17
Cytogenetic Analysis:diploid; relatively unstable
Isoenzymes: G6PD, B
Age: 48 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: Ham's F12K medium, 85%; fetal bovine serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse monolayer once with fresh 0.25% trypsin and let the culture sit for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.