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器官來源：脾
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：HB-204?
運輸方式：凍存運輸
生長狀態(tài)：懸浮生長
數(shù)量：大量
細胞形態(tài)：淋巴樣
免疫類型：mouse IgG1
規(guī)格：0.5mg Designations: LM2/1.6.11
Depositors: ?LJ Miller, TA Springer
Isotype: mouse IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human Mac-1 antigen; against CD11b
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [1617 ]
Comments:Animals were immunized with immunoprecipitates of human granulocyte lysates using TS1/18 (see ATCC HB-203 ) and boosted with live human granulocytes.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with the human Mac-1 antigen and is specific for the alpha chain.
The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost.
Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml.
Subcultivation Ratio: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 1617: Miller LJ, et al. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation. J. Immunol. 137: 2891-2900, 1986. PubMed: 2428876
32897: Zhang L, Plow EF. Overlapping, but not identical, sites are involved in the recognition of C3bi, neutrophil inhibitory factor, and adhesvie ligands by the alpha M beta 2 integrin. J. Biol. Chem. 271: 18211-18216, 1996. PubMed: 8663418