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器官來源：結(jié)腸
細胞類型：上皮細胞
是否是腫瘤細胞：0
物種來源：人
生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
細胞形態(tài)：上皮樣
年限：adult
ATCC Number：CRL-2780?
相關疾?。捍竽c癌
數(shù)量：大量
規(guī)格：0.5mg Designations: ATRFLOX [Mutatect]
Depositors: ?SJ Elledge
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: colon
Disease: colorectal carcinoma
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:molecular studies ataxia telangiectasia and Rad3 related FRAP-related protein-1, Rad3 related protein, protein kinase ATR
DNA Profile (STR):Amelogenin: X
CSF1PO: 7,9
D13S317: 10,12
D16S539: 11,13
D5S818: 10,12
D7S820: 11,12
THO1: 8,9
TPOX: 8
vWA: 17,22
Age: adult
Gender: male
Comments:This cell line is a derivative of ATCC Catalog No. CCL-247 (HCT 116) in which one copy of the ATR (ataxia telangiectasia related) gene has been disrupted, and the other allele has been fixed with lox sites flanking exon 2 making it susceptible to Cre deletion. [PubMed: 11721054].
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 71497: Cortez D, et al. ATR and ATRIP: partners in checkpoint signaling. Science 294: 1713-1716, 2001. PubMed: 11721054