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平臺編號：bio-69053
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國際編號：CRL-2687?
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細(xì)胞信息： LHR-1055
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
LHR-1055
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-2687?
生長狀態(tài)：懸浮生長
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：小鼠
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1; kappa light chain
規(guī)格：0.5mg Designations: LHR-1055
Depositors: ?H Loosfelt, E Milgrom
Isotype: IgG1; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362).
The LHR-29 (ATCC CRL-2685 ), LHR-74 (ATCC CRL-2686 ) and LHR-1055 (ATCC CRL-2687 ) antibodies are additive. They recognize different epitopes.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 56152: Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186
ATCC Number：CRL-2687?
生長狀態(tài)：懸浮生長
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：小鼠
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1; kappa light chain
規(guī)格：0.5mg Designations: LHR-1055
Depositors: ?H Loosfelt, E Milgrom
Isotype: IgG1; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor, extracellular domain
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with recombinant human luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor expressed in E. coli.
Spleen cells were fused with Sp2/0 mouse myeloma cells.
The antibody recognizes the extracellular domain (hormone binding domain) of the LH/hCG receptor, (amino acids domain 21-362).
The LHR-29 (ATCC CRL-2685 ), LHR-74 (ATCC CRL-2686 ) and LHR-1055 (ATCC CRL-2687 ) antibodies are additive. They recognize different epitopes.
The antibodies can be used in Western Blot, ELISA, immunocytochemistry, and immunohistochemistry assays of the human LH/hCG receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 90%; heat-inactivated horse serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
References: 56152: Meduri G, et al. Luteinizing hormone/human chorionic gonadotropin receptors in breast cancer. Cancer Res. 57: 857-864, 1997. PubMed: 9041186
