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運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-2747?
器官來源：腎臟
年限：adult
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
細(xì)胞類型：上皮細(xì)胞
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：其他
規(guī)格：0.5mg Designations: ASK [Atlantic Salmon Kidney]
Depositors: ?J Rolland, J Winton, B Krossoey
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Salmo salar
Morphology:epithelial


Source: Organ: kidney
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Norway
Isolation date: 1998
Age: adult
Gender: female
Comments:The ASK (Atlantic Salmon Kidney) cell line was derived by B. Krossoey in 1998 from cells taken from the normal kidney of an Atlantic Salmon. The cell line may be used to propagate Infectious Salmon Anemia virus (ISAV) of the Atlantic Salmon.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 100%
Temperature: 20.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks.
Interval: Subculture every 10 to 17 days.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Once per week
Preservation: Freeze medium: Complete growth medium supplemented with 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 61187: Devold M, et al. Use of RT-PCR for diagnosis of infectious salmon anaemia virus (ISAV) in carrier sea trout Salmo trutta after experimental infection. Dis. Aquat. Org. 40: 9-18, 2000. PubMed: 10785858