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細(xì)胞類型：其他細(xì)胞類型
運輸方式：凍存運輸
是否是腫瘤細(xì)胞：1
物種來源：人
ATCC Number：CRL-8024?
相關(guān)疾?。焊伟?br /> 數(shù)量：大量
器官來源：肝
生長狀態(tài)：貼壁生長
細(xì)胞形態(tài)：上皮樣 Designations: PLC/PRF/5
Depositors: ?WJ McAleer
Biosafety Level:2 [Cells Contain HEPATITIS B ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: liver
Disease: hepatoma
Cell Type: Alexander cells;
Cellular Products:hepatitis virus B surface antigen (HBsAg)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 13
D5S818: 12
D7S820: 9,11
THO1: 8
TPOX: 8
vWA: 15,16
Comments:The line was originally contaminated with mycoplasma, and was cured by treatment with BM-cycline.
The cells secrete HBsAg.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22189: Monjardino J, Crawford E. Polypeptide profile of HBSAg excreted by a human hepatoma cell line. Virology 96: 652-655, 1979. PubMed: 223321
22198: MacNab GM, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer 34: 509-515, 1976. PubMed: 187208
22236: Skelly J, et al. Hepatitis B surface antigen produced by a human hepatoma cell line. Nature 282: 617-618, 1979. PubMed: 233138
22244: . . Science 209: 407, 1980.
22265: . . S. Afr. J. Med. Sci. 41: 8-9, 1976.
23123: Gilligan A, et al. Variation in EGF-induced EGF receptor downregulation in human hepatoma- derived cell lines expressing different amounts of EGF receptor. Exp. Cell Res. 200: 235-241, 1992. PubMed: 1315281
23525: Alexander JJ, et al. Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J. 50: 2124-2128, 1976. PubMed: 63998