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數量：大量
年限：25 years
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
ATCC Number：CRL-1629?
相關疾?。瑚[狀細胞癌
運輸方式：凍存運輸
器官來源：舌頭
規(guī)格：100 ul Designations: SCC-9
Depositors: ?JG Rheinwald
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:

Source: Organ: tongue
Disease: squamous cell carcinoma
Cellular Products:epidermal keratins; low levels of involucrin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8
THO1: 8,9
TPOX: 9,11
vWA: 17
Age: 25 years
Gender: male
Comments:Growth of SCC-9 is enhanced by using a feeder layer of X-irradiated STO cells (ATCC 56-X).
The cells do not grow well in semi-solid medium.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336