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是否是腫瘤細(xì)胞：1
物種來(lái)源：人
年限：55 years
細(xì)胞形態(tài)：上皮樣
ATCC Number：CRL-1624?
相關(guān)疾?。瑚[狀細(xì)胞癌
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
器官來(lái)源：舌頭
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：1 ml Designations: SCC-4
Depositors: ?JG Rheinwald
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial-like


Source: Organ: tongue
Disease: squamous cell carcinoma
Cellular Products:epidermal keratins; 40 kD keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,13
D16S539: 12
D5S818: 13
D7S820: 9,11
THO1: 9.3
TPOX: 8
vWA: 15,17
Age: 55 years
Gender: male
Comments:SCC-4 forms colonies in semi-solid medium, and is not induced to differentiate by anchorage deprivation.
Clonal growth of these cells is improved by using a 3T3 (ATCC CCL-92 ) feeder layer (see Rheinwald and Green, Cell 6:331, 1975 for methods).
ATCC grows these cells on 56-X, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006.To make the complete growth medium, add the following components to the base medium:

• 400 ng/ml hydrocortisone
• fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Subculture before confluency.
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

• Remove and discard culture medium.
• Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
• Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
• Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
• Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels pre-plated with ATCC 56-X feeder layer (irradiated STO cells).
• Incubate cultures at 37C.

Inoculate new flasks at 3 X 10 exp3 cells per sq. cm.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
feeder layer cells:ATCC 56-X
References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336
26113: Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916