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是否是腫瘤細(xì)胞：1
物種來(lái)源：人
年限：78 years
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來(lái)源：皮膚
ATCC Number：HTB-65?
相關(guān)疾病：惡性黑色素瘤 Designations: MeWo
Depositors: ?Y Kodera
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:fibroblast


Source: Organ: skin
Disease: malignant melanoma
Derived from metastatic site: lymph node
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood type A; HLA A2, A26, Bw16, 18
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,9
D16S539: 10,12
D5S818: 12,13
D7S820: 10,12
THO1: 7,9
TPOX: 8,10
vWA: 15
Isoenzymes: ACP1, B [22551 ]
AK-1, 1 [22551 ]
ES-D, 1 [22551 ]
G6PD, B [22551 ]
GLO-I, 2 [22551 ]
PGM1, 1-2 [22551 ]
PGM3, 1 [22551 ]
Age: 78 years
Gender: male
Ethnicity: White
Comments:The MeWo cell line was initiated by Y. Kodera and M. Bean in 1974 from lymph node tissue.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
23256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 1067619