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平臺編號：bio-69179
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國際編號：CCL-238?
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細胞信息： SW1417
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規(guī)格：Frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
SW1417
器官來源：結腸
細胞形態(tài)：上皮樣
運輸方式：凍存運輸
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
年限：Dukes' type C, grade III
ATCC Number：CCL-238?
數(shù)量：大量
相關疾?。捍竽c癌
規(guī)格：300 ul Designations: SW1417 [SW-1417]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: colon
Tumor Stage: Dukes' type C, grade III
Disease: colorectal adenocarcinoma
Cellular Products:carcinoembryonic antigen (CEA) 362 ng/10 exp6 cells/10 days; keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:No
Oncogene:myc +; ras +; fos +; sis +; p53 -; myb -; abl -; ros -; src -
Antigen Expression:blood type B; Rh+
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1
Age: 53 years
Gender: female
Ethnicity: Caucasian
Comments:CSAp negative (CSAp-).
Colon antigen 3, negative.
The cells are positive for keratin by immunoperoxidase staining.
Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.
The line is positive for expression of c-myc, H-ras, sis and fos oncogenes.
N-myc, K-ras, N-ras and myb oncogene expression were not detected.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
23074: Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345
細胞形態(tài)：上皮樣
運輸方式：凍存運輸
是否是腫瘤細胞：1
物種來源：人
生長狀態(tài)：貼壁生長
年限：Dukes' type C, grade III
ATCC Number：CCL-238?
數(shù)量：大量
相關疾?。捍竽c癌
規(guī)格：300 ul Designations: SW1417 [SW-1417]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: colon
Tumor Stage: Dukes' type C, grade III
Disease: colorectal adenocarcinoma
Cellular Products:carcinoembryonic antigen (CEA) 362 ng/10 exp6 cells/10 days; keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:No
Oncogene:myc +; ras +; fos +; sis +; p53 -; myb -; abl -; ros -; src -
Antigen Expression:blood type B; Rh+
Isoenzymes: ES-D, 1
G6PD, B
PEP-D, 1
PGD, A
PGM1, 1
PGM3, 1
Age: 53 years
Gender: female
Ethnicity: Caucasian
Comments:CSAp negative (CSAp-).
Colon antigen 3, negative.
The cells are positive for keratin by immunoperoxidase staining.
Although the patient from which this cell was derived was blood type B, the cell line does not react with anti type B antibody despite the presence of normal levels of alpha1,3-galactosyltransferase activity.
The line is positive for expression of c-myc, H-ras, sis and fos oncogenes.
N-myc, K-ras, N-ras and myb oncogene expression were not detected.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
23025: Leibovitz A, et al. Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501
23074: Dahiya R, et al. ABH blood group antigen expression, synthesis, and degradation in human colonic adenocarcinoma cell lines. Cancer Res. 49: 4550-4556, 1989. PubMed: 2545345
