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年限：13 year old
細(xì)胞形態(tài)：混合型
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：1
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
ATCC Number：CRL-8303?
器官來源：骨
相關(guān)疾?。汗侨饬?br /> 規(guī)格：80 ml Designations: 143B
Depositors: ?Wistar Institute
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:mixed


Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18
Age: 13 year old
Gender: female
Ethnicity: Caucasian
Comments:Thymidine kinase negative (TK-).
This is a human osteosarcoma cell line.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10%
Temperature: 37.0℃
Subculturing: Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Preservation: 90% FBS; 10% DMSO
Related Products:derivative:ATCC CRL-8304
References: 32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256
32519: Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 8627758
33047: Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 8662757
33152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996. PubMed: 8702651