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生長狀態(tài)：貼壁生長
運輸方式：凍存運輸
ATCC Number：HTB-88?
相關(guān)疾病：其他疾病
細胞形態(tài)：成纖維樣
是否是腫瘤細胞：1
物種來源：人
器官來源：其他
年限：43 years
數(shù)量：大量
規(guī)格：60 ml Designations: SK-LMS-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: vulva
Disease: leiomyosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Isolation: Isolation date: 1971
Applications:transfection host (Roche Transfection Reagents )
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 9,10
D13S317: 12
D16S539: 8,11
D5S818: 11,13
D7S820: 8,9
THO1: 6,7
TPOX: 8,9
vWA: 18,20
Cytogenetic Analysis:The cell line is aneuploid human female, with chromosome counts in the triploid to hypertriploid range. Neither of the X chromosomes are normal, but their q arms are detected in a translocation with chromosome N4. Normal chromosomes N1, N4, N13, and N17 are clearly under-represented, while chromosomes N6, N18, and N20 are consistently over-represented with respect to the copy numbers of other normal chromosomes. Eight marker chromosomes are identified: 5p+, t(1pter--->cen::9q12--->9q33::3p21--->3pter), t(1p;?), der(2)t(1;2)(q21;q24), 12p+, der(6)t(6;?)(q21;?), der(4)t(X;4)(q13;q34), del(16)(p13). The cytogenetic and isozyme phenotype descriptors are in accord with those given by J. Fogh.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 1-2
Age: 43 years
Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
32274: Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
32289: Hu M, et al. Purification and characterization of human lung fibroblast motility-stimulating factor for human soft tissue sarcoma cells: identification as an NH2-terminal fragment of human fibronectin. Cancer Res. 57: 3577-3584, 1997. PubMed: 9270031