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是否是腫瘤細(xì)胞：1
物種來源：人
器官來源：眼
生長狀態(tài)：懸浮生長
ATCC Number：HTB-18?
數(shù)量：大量
相關(guān)疾?。浩渌膊?br /> 細(xì)胞形態(tài)：其他
運(yùn)輸方式：凍存運(yùn)輸
組織來源：retina
年限：2.5 years
規(guī)格：150 μl Designations: Y79
Depositors: ?B Gallie
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:multicellular clusters
Source: Organ: eye
Tissue: retina
Disease: retinoblastoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript:negative
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 15,18
Cytogenetic Analysis:hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Age: 2.5 years
Gender: female
Ethnicity: Caucasian
Comments:The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation.
The donor had a strong maternal family history of retinoblastoma.
Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22531: Reid TW, et al. Characteristics of an established cell line of retinoblastoma. J. Natl. Cancer Inst. 53: 347-360, 1974. PubMed: 4135597
32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
32684: Wong HK, Ziff EB. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J. Virol. 70: 332-340, 1996. PubMed: 8523545