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細(xì)胞形態(tài)：上皮樣
年限：47 years adult
器官來源：其他
運(yùn)輸方式：凍存運(yùn)輸
生長狀態(tài)：貼壁生長
數(shù)量：大量
ATCC Number：HTB-105?
相關(guān)疾病：其他疾病
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：10 μg Designations: Tera-1
Depositors: ?J Fogh
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: testes
Disease: embryonal carcinoma, malignant
Derived from metastatic site: lung
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic:No
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 12
D16S539: 10,12
D5S818: 11,13
D7S820: 9,10
THO1: 7,8
TPOX: 8
vWA: 14
Cytogenetic Analysis:at passage 20, hypotriploid (+A, +A3, +B, +C, +D, +E, +F, -A2) with abnormalities including endoreduplications, acrocentric fragments, minutes, translocations, secondary constrictions, breaks and large metacentric markers
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1-2
Age: 47 years adult
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Subculture every 6 to 8 days.
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
32279: Schar BK, et al. Simultaneous detection of all four alkaline phosphatase isoenzymes in human germ cell tumors using reverse transcription-PCR. Cancer Res. 57: 3841-3846, 1997. PubMed: 9288797