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ATCC Number：CRL-7691?
相關(guān)疾病：惡性黑色素瘤
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：1
物種來源：人
細(xì)胞形態(tài)：成纖維樣
器官來源：皮膚
數(shù)量：大量
年限：57 years
運(yùn)輸方式：凍存運(yùn)輸 Designations: Hs 940.T
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: skin
Disease: malignant melanoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis:modal number = 46; range = 43 to 92
Age: 57 years
Gender: male
Ethnicity: Caucasian
Comments:Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

70%密度的25cm2培養(yǎng)瓶的細(xì)胞一瓶特點(diǎn)：細(xì)胞代數(shù)為2-3代包裝：內(nèi)層無菌自封袋；專用防壓泡沫盒；外層防壓保溫氣泡袋；說明書細(xì)胞來源：ATCC貨期：1-2周運(yùn)輸方式：快遞運(yùn)輸售后：收到細(xì)胞后7天，如發(fā)現(xiàn)細(xì)胞有質(zhì)量問題（如污染，死亡，等）請及時(shí)聯(lián)系