年限:stage E
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
是否是腫瘤細(xì)胞:1
物種來(lái)源:人
ATCC Number:CRL-5855?
數(shù)量:大量
相關(guān)疾?。盒〖?xì)胞肺癌
運(yùn)輸方式:凍存運(yùn)輸
器官來(lái)源:肺
規(guī)格:100 ul                                                                                                                                                                                                                                                                                                                                                          Designations: NCI-H1092 [H1092]
Depositors:                  ?AF Gazdar, JD Minna 
Biosafety Level:1
Shipped:        frozen 
Medium & Serum: See Propagation          Growth        Properties:suspension
Organism: 
Homo sapiens
Morphology: 
Source: 
Organ:  lung 
Tumor Stage:  stage E 
Disease:  carcinoma; classic small cell lung          cancer 
Derived from metastatic site:  bone marrow
Permits/Forms:In addition to the MTA          mentioned above, other ATCC            and/or regulatory permits  may be required for the          transfer of this ATCC material. Anyone purchasing ATCC          material is ultimately responsible for obtaining the permits.          Please click here  for information regarding          the specific requirements for shipment to your location.
Restrictions:The line is available with the          following restrictions: 1. This cell line was deposited at the          ATCC by Dr. A. Gazdar and Dr. J. Minna and is provided for          research purposes only. Neither the  cell line nor products          derived from it may be sold or used for commercial purposes.          Nor can the cells be distributed to third parties for purposes          of sale, or producing for sale, cells or their products. The          cells are provided as  service to the research community. They          are provided without warranty of merchantability or fitness          for a particular purpose or any other warranty, expressed or          implied. 2. Any proposed commercial use of the these cells, or          their  products must first be negotiated with the University          of Texas Southwestern Medical Center at Dallas, 5323 Harry          Hines Blvd., Dallas, Texas 75235. Telephone (214) 699-8056,          FAX (214) 688-7233.
Cytogenetic        Analysis:del(p12-p23)
Age:        67 years 
Gender:        male 
Ethnicity:        Caucasian 
Comments:The line was established in June 1985. 
Fast growing. 
The patient was a smoker. 
120 pack years.
Propagation: 
 ATCC complete growth medium:  HITES medium          supplemented with 5% fetal bovine serum
	The base medium for this cell line is ATCC-formulated            DMEM:F12 Medium Catalog No.30-2006. To make the complete            growth medium,add the following components to the base              medium
	- 
		0.005 mg/ml Insulin
	
 
	- 
		0.01 mg/ml Transferrin
	
 
	- 
		30nM Sodium selenite (final conc.)
	
 
	- 
		10 nM Hydrocortisone (final conc.)
	
 
	- 
		10 nM beta-estradiol (final conc.)
	
 
	- 
		extra 2mM L-glutamine (for final conc. of 4.5 mM)
	
 
	- 
		5% fetal bovine serum (final conc.)
	
 
Preservation:        Culture medium, 95%; DMSO, 5%
Related        Products:Recommended medium (without the          additional supplements or serum described under ATCC          Medium):ATCC 30-2006
References:           23570: . NCI-Navy Medical Oncology Branch          Cell Line Supplement.  J. Cell. Biochem. suppl. 24: 1996..