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平臺(tái)編號(hào)：bio-69285
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國(guó)際編號(hào)：CRL-2500?
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細(xì)胞信息： A7
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
A7
ATCC Number：CRL-2500?
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。汉谏亓?br /> 數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：其他
器官來(lái)源：皮膚
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
規(guī)格：200次 Designations: A7 [M2A7]
Depositors: ?TP Stossel
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:melanocytic
Source: Organ: skin
Disease: melanoma
Cellular Products:filamin-1 (actin-binding protein ABP or ABP-280)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:M2 cells are severely deficient in filamin-1 mRNA and protein. Filamin-1, also known as actin-binding protein (ABP, APB-280), cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins. [42092 ]
The filament-1 gene on the X chromosome is present in these cells.
ABP (filamin-1) was transfected into M2 cells in a mammalian expression vector (LK444) containing full length human ABP recombinant DNA (LK-APB). [42092 ]
A7 cells express levels of filamin-1 comparable to wild-type filamin-1 containing melanoma cells. [42092 ]
Filamin-1 is essential for cell migration and has been shown to bind partners in cell signal transduction, cell membrane sorting and cytoskeleton-membrane association.
A7 cells are useful as a control for studying these systems.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 10 mM HEPES, 0.5 mg/ml G418 and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin - 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 42092: Cunningham CC, et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 255: 325-327, 1992. PubMed: 1549777
43261: Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J. Cell Biol. 129: 1589-1599, 1995. PubMed: 7790356
43263: Cantiello HF, et al. Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells. J. Biol. Chem. 268: 4596-4599, 1993. PubMed: 8383118
47049: Byers HR, et al. Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. Am. J. Pathol. 139: 423-435, 1991. PubMed: 1867326
運(yùn)輸方式：凍存運(yùn)輸
相關(guān)疾?。汉谏亓?br /> 數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞形態(tài)：其他
器官來(lái)源：皮膚
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
規(guī)格：200次 Designations: A7 [M2A7]
Depositors: ?TP Stossel
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:melanocytic
Source: Organ: skin
Disease: melanoma
Cellular Products:filamin-1 (actin-binding protein ABP or ABP-280)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:M2 cells are severely deficient in filamin-1 mRNA and protein. Filamin-1, also known as actin-binding protein (ABP, APB-280), cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins. [42092 ]
The filament-1 gene on the X chromosome is present in these cells.
ABP (filamin-1) was transfected into M2 cells in a mammalian expression vector (LK444) containing full length human ABP recombinant DNA (LK-APB). [42092 ]
A7 cells express levels of filamin-1 comparable to wild-type filamin-1 containing melanoma cells. [42092 ]
Filamin-1 is essential for cell migration and has been shown to bind partners in cell signal transduction, cell membrane sorting and cytoskeleton-membrane association.
A7 cells are useful as a control for studying these systems.
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 10 mM HEPES, 0.5 mg/ml G418 and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin - 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 42092: Cunningham CC, et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 255: 325-327, 1992. PubMed: 1549777
43261: Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J. Cell Biol. 129: 1589-1599, 1995. PubMed: 7790356
43263: Cantiello HF, et al. Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells. J. Biol. Chem. 268: 4596-4599, 1993. PubMed: 8383118
47049: Byers HR, et al. Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. Am. J. Pathol. 139: 423-435, 1991. PubMed: 1867326
