-
平臺(tái)編號(hào)：bio-69287
-
國(guó)際編號(hào)：CRL-1547?
-
細(xì)胞信息： MNNG/HOS (Cl #5)
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
MNNG/HOS (Cl #5)
年限：13 years
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：骨
ATCC Number：CRL-1547?
相關(guān)疾?。汗侨饬?br /> 生長(zhǎng)狀態(tài)：貼壁生長(zhǎng) Designations: MNNG/HOS Cl #5 [R-1059-D]
Depositors: ?JS Rhim
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Isoenzymes: G6PD, B
Age: 13 years
Gender: female
Ethnicity: Caucasian
Comments:This line was derived from HOS (ATCC CRL-1543 ) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.
References: 22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046
26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298
32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：骨
ATCC Number：CRL-1547?
相關(guān)疾?。汗侨饬?br /> 生長(zhǎng)狀態(tài)：貼壁生長(zhǎng) Designations: MNNG/HOS Cl #5 [R-1059-D]
Depositors: ?JS Rhim
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:
Source: Organ: bone
Disease: osteosarcoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18
Isoenzymes: G6PD, B
Age: 13 years
Gender: female
Ethnicity: Caucasian
Comments:This line was derived from HOS (ATCC CRL-1543 ) by transformation with 0.01 mcg/ml MNNG (a carcinogenic nitrosamine). The cells exhibit a high saturation density, a high plating efficiency in soft agar and are tumorigenic in nude mice.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 2 to 5 days.
References: 22895: Rhim JS, et al. Transformation of human cells in culture by N-methyl-N'-nitro-N- nitrosoguanidine. Nature 256: 751-753, 1975. PubMed: 1057046
26084: Rhim JS, et al. Characterization of human cells transformed in vitro by N-methyl-N'- nitro-N-nitrosoguanidine. Int. J. Cancer 19: 505-510, 1977. PubMed: 265298
32925: Zhu X, et al. Cell cycle-dependent modulation of telomerase activity in tumor cells. Proc. Natl. Acad. Sci. USA 93: 6091-6095, 1996. PubMed: 8650224
