年限：72 years
ATCC Number：HTB-12?
數(shù)量：大量
相關(guān)疾病：其他疾病
器官來源：大腦
細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
生長狀態(tài)：貼壁生長
是否是腫瘤細(xì)胞：1
物種來源：人
規(guī)格：100 ul Designations: SW 1088 [SW-1088, SW1088]
Depositors: ?A Leibovitz
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: brain
Disease: astrocytoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood type A; Rh+
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 7,9.3
TPOX: 8
vWA: 16,17
Cytogenetic Analysis:hypertriploid; modal number = 72 to 74. The rate of higher ploidies was 4.2%. Most chromosomes were morphologically normal. Three marker chromosomes were common to all cells: del(1) (q11), der (9)t(7;?;9) (q11?;?;p24), and der (10)t(4;10) (q21;q15)., The der (9) was paired in nearly 50% of the cells. Usually one, but occasionally three double minutes (DM) were seen in a few cells. Five copies of normal N5, N7 and N20 were seen in most cells., The X and Y were paired. The presence of Y chromosomes was confirmed in the QM stained preparation.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1-2
PGM1, 1-2
PGM3, 1
Age: 72 years
Gender: male
Ethnicity: Caucasian
Comments:The SW 1088 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1975 from an astrocytoma taken from a 72 year old male Caucasian. The cell line was received at the ATCC in January, 1982 at passage 23.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
22551: Wright WC, et al. Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
