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年限：45 years
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
器官來(lái)源：皮膚
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
ATCC Number：CRL-1974?
相關(guān)疾病：惡性黑色素瘤
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
規(guī)格：48T Designations: COLO 829
Depositors: ?GE Moore
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: skin
Disease: malignant melanoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19
Age: 45 years
Gender: male
Ethnicity: Caucasian
Comments:The COLO 829 cell line was established by G.E. Moore from a biopsy specimen taken from a patient prior to chemotherapy for metastatic melanoma.
A cell line was also produced from peripheral blood B cells, and is available as COLO 829BL (ATCC CRL-1980 ).
Early passages of COLO 829 still contain a small percentage of melanin producing cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
normal (or near-normal) cell line established from the same patient:ATCC CRL-1980