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數量：大量
生長狀態(tài)：貼壁生長
器官來源：乳房
年限：TNM stage IIA, grade 3
運輸方式：凍存運輸
ATCC Number：CRL-2320?
相關疾?。簩Ч馨?br /> 組織來源：duct
細胞形態(tài)：上皮樣
是否是腫瘤細胞：1
物種來源：人
規(guī)格：100 ul Designations: HCC1008
Depositors: ?AF Gazdar, AK Virmani
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: mammary gland; breast
Tissue: duct
Tumor Stage: TNM stage IIA, grade 3
Disease: ductal carcinoma
Derived from metastatic site: lymph node
Cellular Products:Epithelial glycoprotein 2 (EGP2)
cytokeratin 19
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The line is available with the following restrictions: 1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied. 2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.
Isolation: Isolation date: June 7, 1994
Applications:The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
The cells are positive for expressions of Her2-neu and p53 oncogenes.
HCC1008 is positive for the epithelial cell specific marker, Epithelial Glycoprotein 2 (EGP2) and cytokeratin 19.
Receptors:estrogen receptor, not expressed
progesterone receptor, not expressed
Oncogene:her2/neu +, p53 +
DNA Profile (STR):Amelogenin: X
CSF1PO: 12,13
D13S317: 12
D16S539: 14,15
D5S818: 13
D7S820: 10
THO1: 7
TPOX: 11
vWA: 17,19
Cytogenetic Analysis:multiploid; cell population has several ploidy indices; double minute (DM) chromosomes were observed
Age: 67 years adult
Gender: female
Ethnicity: Black
Comments:This cell line was initiated from an axillary lymph node on 6/7/94 and took 12.5 months to establish.
The tumor was classified as TNM stage IIA, grade 3, ductal carcinoma with 12 out of 12 lymph node metastasis.
The cells are positive for expressions of Her2-neu and p53 oncogenes.
HCC1008 is positive for the epithelial cell specific marker, Epithelial Glycoprotein 2 (EGP2) and cytokeratin 19.
The cells are negative for expression of estrogen receptor (ER) and for expression of progesterone receptor (PR).
Propagation: ATCC complete growth medium: HITES medium supplemented with 10% fetal bovine serum.The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No.30-2006. To make the complete growth medium,add the following components to the base medium:

• 0.005 mg/ml Insulin
• 0.01 mg/ml Transferrin
• 30nM Sodium selenite (final conc.)
• 10 nM Hydrocortisone (final conc.)
• 10 nM beta-estradiol (final conc.)
• extra 2mM L-glutamine (for final conc. of 4.5 mM)
• 10% fetal bovine serum (final conc.)

Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: This cell line grows exceedingly slow. Cells grow in patches and cultures only become 50 to 60% confluent.
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Add 3.0 to 4.0 ml Cell Dissociation Buffer (GIBCO #13150-016) to cell layer and incubate at room temperature or 37C.
3. Observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 10 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. To remove Cell Dissociation Buffer, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.
6. Discard supernatant and resuspend cell pellet in fresh complete growth medium. Aspirate cells with a small bore pipette. Add appropriate aliquots of cell suspension to new culture vessels.
7. Place culture vessels in incubators at 37?C.

Cells reattach slowly after subculture (about 50% attached and 50% in suspension). Allow flasks to remain undisturbed for at least a week for cultures to become reestablished. It can take as along as three to four weeks before cultures can be subcultured again.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
Medium additive:ATCC 30-2214
normal (or near-normal) cell line established from the same patient:ATCC CRL-2319
purified DNA:ATCC CRL-2320D
purified RNA:ATCC CRL-2320R
References: 38266: Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771