年限：grade 3, stage IIIC
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：上皮樣
ATCC Number：CRL-11732?
器官來(lái)源：卵巢
相關(guān)疾?。浩渌膊?br />
是否是腫瘤細(xì)胞：1
物種來(lái)源：人
規(guī)格：10 μg Designations: OV-90
Depositors: ?University of Montreal
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:epithelial
Source: Organ: ovary
Tumor Stage: grade 3, stage IIIC
Disease: malignant papillary serous adenocarcinoma
Derived from metastatic site: ascites
Cellular Products:keratin [49408 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: August, 1992
Tumorigenic:Yes
Oncogene:her2/neu +, p53 (mutated, Ser --> Arg mutation at exon 6, codon 215)
DNA Profile (STR):Amelogenin: X
CSF1PO: 12,13
D13S317: 11,12
D16S539: 11
D5S818: 11,15
D7S820: 10,10.1
THO1: 9.3
TPOX: 8,10
vWA: 16,17
Cytogenetic Analysis:46, XX, der(1)t(1;10)(p36;p15), hsr(3)(p11), der(9;17)(q10;q10), der(10)t(10;17)(p15;p12p13), der(13)t(13;13)(p11;q14) [49408 ]
Age: 64 years
Gender: female
Comments:This cell line was initiated in August of 1992 from a patient of French-Canadian descent with no family history of ovarian cancer. [49408 ]
Like TOV-21G (ATCC CRL-11730 ), the OV-90 (ATCC CRL-11732 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24. [42090 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 15%
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References: 42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993
49722: Lounis H, et al. Mapping of chromosome 3p deletions in human epithelial ovarian tumors. Oncogene 17: 2359-2365, 1998. PubMed: 9811467
