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生長狀態(tài)：貼壁生長
數(shù)量：大量
是否是腫瘤細(xì)胞：1
物種來源：人
年限：53 year old adult
ATCC Number：CRL-1585?
器官來源：皮膚
相關(guān)疾?。浩渌膊?br /> 運(yùn)輸方式：凍存運(yùn)輸 Designations: C32
Depositors: ?TR Chen
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:

Source: Organ: skin
Disease: melanoma, amelanotic
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 9
vWA: 14,19
Age: 53 year old adult
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
derivative:ATCC CRL-1579
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
References: 26091: Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716
26092: Chen TR, Shaw MW. Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368