-
平臺編號：bio-69370
-
國際編號：CRL-2289?
-
細胞信息： DB
-
規(guī)格：Frozen
-
用途：ATCC原裝細胞
-
服務費用：
加載中…… - 訂購
-
注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
DB
ATCC Number：CRL-2289?
相關疾?。浩渌膊?br /> 細胞類型：B淋巴細胞
細胞形態(tài)：淋巴樣
是否是腫瘤細胞：1
物種來源：人
免疫類型：IgG; lambda light chain
年限：45 years
運輸方式：凍存運輸
數(shù)量：大量
生長狀態(tài)：懸浮生長
規(guī)格：60 次 Designations: DB
Depositors: ?M Beckwith
Isotype: IgG; lambda light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: large cell lymphoma
Cell Type: B lymphoblast;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 11
THO1: 6,8
TPOX: 8
vWA: 15,16
Age: 45 years
Gender: male
Ethnicity: Caucasian
Comments:The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma.
The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor.
Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition.
They are reported to be negative for the Epstein-Barr virus genome.
The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 to 8 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
相關疾?。浩渌膊?br /> 細胞類型：B淋巴細胞
細胞形態(tài)：淋巴樣
是否是腫瘤細胞：1
物種來源：人
免疫類型：IgG; lambda light chain
年限：45 years
運輸方式：凍存運輸
數(shù)量：大量
生長狀態(tài)：懸浮生長
規(guī)格：60 次 Designations: DB
Depositors: ?M Beckwith
Isotype: IgG; lambda light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: large cell lymphoma
Cell Type: B lymphoblast;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 -
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 11
THO1: 6,8
TPOX: 8
vWA: 15,16
Age: 45 years
Gender: male
Ethnicity: Caucasian
Comments:The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma.
The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor.
Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition.
They are reported to be negative for the Epstein-Barr virus genome.
The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 to 8 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
