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運輸方式：凍存運輸
數(shù)量：大量
年限：43 years
生長狀態(tài)：貼壁，多細胞聚集
ATCC Number：CCL-250.1?
相關(guān)疾?。捍竽c癌
是否是腫瘤細胞：1
物種來源：人
器官來源：盲腸
細胞形態(tài)：上皮樣
規(guī)格：100 ul Designations: SNU-C2A
Depositors: ?AF Gazdar
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:loosely adherent, multicell aggregates
Organism: Homo sapiens
Morphology:epithelial


Source: Organ: cecum
Disease: colorectal carcinoma
Cellular Products:carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood type A; Rh+
Cytogenetic Analysis:modal number = 46; range = 44 to 48.
This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 86%, with polyploidy at 16.2%. The ins(1;?)(p13;?), del(3)(p23), t(4q,?), t(8;?), del(9)(p13), del(11)(p13), 14q+ and two other marker chromosomes are common to all metaphases examined. Other altered chromosomes, if any, occur only sporadically. Normal N11 is absent. Two copies of X chromosomes are found in each cell. The Y chromosome is not found in QM stained preparations.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 2
PGM1, 1
PGM3, 1
Age: 43 years
Gender: female
Ethnicity: Asian
Comments:This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.
The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.
The line was initially grown in RPMI 1640 medium with 20% fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4 (it can also be grown in medium with 10% serum).
The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.
Propagation: ATCC complete growth medium: ACL-4 medium supplemented with 10% FBS
The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

1. 0.02 mg/ml insulin
2. 0.01 mg/ml transferrin
3. 25 nM sodium selenite
4. 50 nM Hydrocortisone
5. 1 ng/ml Epidermal Growth Factor (do not filter)
6. 0.01 mM ethanolamine
7. 0.01 mM phosphorylethanolamine
8. 100 pM triiodothyronine
9. 0.5% (w/v) bovine serum albumin
10. 10 mM HEPES
11. 0.5 mM sodium pyruvate
12. 2mM L-glutamine(for final conc. of 4.5 mM)
13. 10% FBS

Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Growth is slow.
Subculturing: Protocol: Cultures can be maintained by scraping off the attached cells and transfer along with the the floating cells into new flasks.
Medium Renewal: Two times weekly
Preservation: Freeze medium: Complete growth medium supplemented with an additonal 10% fetal bovine serum and 7.5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249