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平臺編號：bio-69388
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國際編號：HTB-60?
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細(xì)胞信息： EB1
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規(guī)格：Frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
EB1
年限：9 years
細(xì)胞形態(tài)：淋巴樣
ATCC Number：HTB-60?
相關(guān)疾病：淋巴瘤
組織來源：upper maxilla
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：1
物種來源：人
數(shù)量：大量
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：100次 Designations: EB1
Depositors: ?G Trempe, LJ Old
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: upper maxilla
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Age: 9 years
Gender: female
Ethnicity: Black
Comments:The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma.
Viral particles (Epstein-Barr virus) were noted through electron microscopic examination.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Allow cells to settle, remove most of the supernatant medium, dilute with fresh medium to 4 X 10 exp5 viable cell/ml. Subculture at time of fluid renewal.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
22169: Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790
22722: . . Br. J. Cancer 19: 108-115, 1965.
23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014
26323: . . J. Exp. Med. 121: 761-770, 1965.
26324: Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357
26325: Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453
26326: Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. PubMed: 6818551
細(xì)胞形態(tài)：淋巴樣
ATCC Number：HTB-60?
相關(guān)疾病：淋巴瘤
組織來源：upper maxilla
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：1
物種來源：人
數(shù)量：大量
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：100次 Designations: EB1
Depositors: ?G Trempe, LJ Old
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Tissue: upper maxilla
Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isoenzymes: ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Age: 9 years
Gender: female
Ethnicity: Black
Comments:The EB1 line was isolated in 1963 by M.A. Epstein and Y.M. Barr from biopsy fragments and cell clumps of the lymphoma.
Viral particles (Epstein-Barr virus) were noted through electron microscopic examination.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Allow cells to settle, remove most of the supernatant medium, dilute with fresh medium to 4 X 10 exp5 viable cell/ml. Subculture at time of fluid renewal.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22143: Epstein MA, Barr YM. Cultivation in vitro of human lymphoblasts from Burkitt's malignant lymphoma. Lancet 1: 252-253, 1964. PubMed: 14090852
22169: Epstein MA, Barr YM. Characteristics and mode of growth of tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J. Natl. Cancer Inst. 34: 231-240, 1965. PubMed: 14293790
22722: . . Br. J. Cancer 19: 108-115, 1965.
23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014
26323: . . J. Exp. Med. 121: 761-770, 1965.
26324: Leder P, et al. Translocations among antibody genes in human cancer. Science 222: 765, 1983. PubMed: 6356357
26325: Dalla-Favera R, et al. Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc. Natl. Acad. Sci. USA 79: 7824-7827, 1982. PubMed: 6961453
26326: Taub R, et al. Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc. Natl. Acad. Sci. USA 79: 7837-7841, 1982. PubMed: 6818551
